Just wondering: Does it make a difference, performance-wise, between the following two cases?

Case 1:
Tablespace A with single datafile on a physical disk

Case 2:
Tablespace A with multiple datafiles on the SAME SINGLE physical disk

Of course, we should spread out the datafiles as much as we can but if I only have one physical disk, does it make a difference whether I have multiple datafiles?

Thanks,